You can do that with a subquery. Please find an example below. The subquery lists computers having this account and you are listing computers in the main report which don't get listed by the subquery. Not sure if added the filter tblUsers.SID Like '%-500' by intention? This would filter on built-in administrator accounts only.
Select Top 1000000 tblAssets.AssetID,
tblAssets.AssetUnique,
tblAssets.Domain,
tsysOS.OSname,
tblAssets.Description,
tsysOS.Image As icon
From tblAssets
Inner Join tblAssetCustom On tblAssets.AssetID = tblAssetCustom.AssetID
Inner Join tsysOS On tblAssets.OScode = tsysOS.OScode
Where tblAssets.AssetID Not In (Select tblUsers.AssetID From tblUsers
Where tblUsers.Name = 'saveme') And
tblAssetCustom.State = 1
Order By tblAssets.AssetName